LOCUS       ISCR1-bleMBL-blaNDM-1_unit5309 bp   DNA    linear       03-OCT-2020
DEFINITION  Providencia sp.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Providencia sp.
  ORGANISM  Providencia sp.
            Unclassified.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-bleMBL-blaNDM-1 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Providencia sp."
                     /mol_type="genomic DNA"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /product="ISCR1 transposase"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     misc_recomb     2134..2154
                     /gene="oriIS"
     misc_feature    complement(2153..2990)
                     /gene="truncated dsbD"
                     /product="Truncated oxidoreductase superfamily protein
                     (pseudogene)"
     CDS             complement(3001..3639)
                     /gene="trpF"
                     /codon_start=1
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVA
                     GALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKP
                     VWKALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALP
                     WGLAGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
     CDS             complement(3644..4009)
                     /gene="bleMBL"
                     /codon_start=1
                     /product="Bleomycin resistance protein"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQ
                     LEFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLR
                     IGYLIDPDCTLVRLIQNPD"
     CDS             complement(4013..4825)
                     /gene="blaNDM-1"
                     /codon_start=1
                     /product="Beta-lactamase NDM-1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQ
                     RFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQIL
                     NWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHS
                     LTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSL
                     GNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
     repeat_region   complement(4919..4944)
                     /gene="IRR_ISAba125"
     misc_feature    complement(4926..5309)
                     /gene="truncated tnpA"
                     /product="Truncated ISAba125 transposase (pseudogene)"
ORIGIN      
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat acccggcacc
     2161 gcacctcggt caaggccgat gccgccttca ccggcgcgac catggcgctg ccgaagggca
     2221 aggcctaccg cgacgaattc ttcggcgacg tcgaaaccta ccgcaaggaa ttgctcggca
     2281 ccctcaccgg cacgcccgcg gccggcgcga gcgcgaccac cctgaccgtg aagtaccagg
     2341 gctgcgccga tgccggcgtg tgctacccgc cgcagacccg caccctgaag gtcgcgttgc
     2401 cgggcgaagc gggcgctggc ggcttcggct gtaaggcgcg cgggctgcat gattatgtcg
     2461 tcaagaacgg ctctgccgat catcccaacg ccgaggtgaa attcgcactg ggtgatgtgg
     2521 tcaacaccat gatcggctgc actaatggtg aaacgatcat gctgtgccac gacacctcgc
     2581 tgccgcgccc ctattctctc ggctttcggg tgcaaggcac cgaggggctg tggatggacg
     2641 tcaacaagtc gatctatctg gagggcaaga gcccacagcc gcaccgctgg gagcctgccg
     2701 agggctggtt tgcgaaatac gatcacccgc tatggaaacg ctacgccgat ctggcggcag
     2761 gggccgggca tggcgggatg gactggttcg tgatccacgc ttttgtcgag gcgctgaagg
     2821 ccaaggcccc gatgccaatc gacatttacg acgcgctggc ctggagcgcg atcacgcctc
     2881 tgtcggaaca atcgattgct gagggcaatc gcacgttaga ttttcccgac ttcacccgag
     2941 ggcagtggcg cacccgcaag ccgatctttg cgctgaacga cgcctattga tcgacgcgat
     3001 ttaggccaag cgcaccgcaa aggcgaaatt ggtaatcttg tcggtatcct tgacgcccgg
     3061 cgcgctttcg acgccgctgg aggtatcgac cagcggcgct ccggtgcgcg caatcgcctc
     3121 ggcaacattc gtcggattta gcccgcctgc cagcccccac ggcaaggcac cgcgatatcc
     3181 ggccagcagc gaccagtcga acgccaaccc catgccgccg ggcagcgcgc ctttgggggt
     3241 cttggcgtcg aacaagatca agtccgccgc cccggcatag gctgcggcgc gtgcgacatc
     3301 gctggcgctg gcgacgggca gcgccttcca caccggcttg ccaaaccgcg cgcgcaactg
     3361 ggccacgcgt tcgggcgatt ccgaaccgtg cagctgcagc gcgttcagct tggctgccac
     3421 cagtgcgtcg gcgatgacag catcatccgc atcgacgaac aaaccgacca tggcgatctg
     3481 gccagctgcg cgcgatgtca aagcgcccgc gacattcgac gtaaccgcac ggggcgacgc
     3541 tggatagaac accaacccgg catagtccgc ccgcgccgcg atggtcgcat cgagcgcctc
     3601 gggtgtgctg atcccgcaaa tcttgatttt cgcgggcatg cggtcagtcg gggttctgga
     3661 tcagccgcac cagcgtgcag tcgggatcga tcaggtagcc gatcctcagg ccgctcgcct
     3721 ccagttgcgg agctttgaag cgcggccagc cggtgctttt ttcctcggct cccgccgcgt
     3781 tcaccaatgc caccatggca tcgagatcat ccaaccgcag gcaacagccg aacgagctcg
     3841 tagctgggtc gaggtcagga taggggaaga attcgagctg caaaccgccg cgctgcagga
     3901 tcatccagcc gcgatccttc caactcgtcg caaagcccag cttcgcataa aacgcctctg
     3961 tcacatcgaa atcgcgcgat ggcagattgg gggtgacgtg gtcagccatg gctcagcgca
     4021 gcttgtcggc catgcgggcc gtatgagtga ttgcggcgcg gctatcgggg gcggaatggc
     4081 tcatcacgat catgctggcc ttggggaacg ccgcaccaaa cgcgcgcgct gacgcggcgt
     4141 agtgctcagt gtcggcatca ccgagattgc cgagcgactt ggccttgctg tccttgatca
     4201 ggcagccacc aaaagcgatg tcggtgccgt cgatcccaac ggtgatattg tcactggtgt
     4261 ggccggggcc ggggtaaaat accttgagcg ggccaaagtt gggcgcggtt gctggttcga
     4321 cccagccatt ggcggcgaaa gtcaggctgt gttgcgccgc aaccatcccc tcttgcgggg
     4381 caagctggtt cgacaacgca ttggcataag tcgcaatccc cgccgcatgc agcgcgtcca
     4441 taccgcccat cttgtcctga tgcgcgtgag tcaccaccgc cagcgcgacc ggcaggttga
     4501 tctcctgctt gatccagttg aggatctggg cggtctggtc atcggtccag gcggtatcga
     4561 ccaccagcac gcggccgcca tccctgacga tcaaaccgtt ggaagcgact gccccgaaac
     4621 ccggcatgtc gagataggaa gtgtgctgcc agacattcgg tgcgagctgg cggaaaacca
     4681 gatcgccaaa ccgttggtcg ccagtttcca tttgctggcc aatcgtcggg cggatttcac
     4741 cgggcatgca cccgctcagc atcaatgcag cggctaatgc ggtgctcagc ttcgcgaccg
     4801 ggtgcataat attgggcaat tccatcaagt tttcctttta ttcagcatta aaaaccccgc
     4861 aaatgcgagg cctagtaaat agatgatctt aatttggttc actgtagcaa aaatatgggg
     4921 cgaattcaaa catgaggtgc gacagtttca aaagccatat gataatcaac aagctgagca
     4981 aatttctcta atggtgtaag ccaatctaac gcctttctag gacgagtatt cagtgacatg
     5041 gcaacttgat ttaaataatg ctgatctgcc tgatttaaat caatcccttt aggtaaatat
     5101 tgcctaatta aaccattcat attttcgcat gtgccttttt gccagggtga atgtgggtca
     5161 cagaaatata catctatgcc taaatcttct tcgagtattt tatgttctga catctcacgt
     5221 ccacggtcat aggtcaacgt tttacgcagt tctgcaggta aatatttcag agcttcagtt
     5281 aaagccttgc gcactgattc tgcctttgc
//
